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KMID : 0613819950050020063
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Journal of Life Science
1995 Volume.5 No. 2 p.63 ~ p.69
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Detection of cockroach(blattella germanica L.) specific allrgens by monoclonal natibodies
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Abstract
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Cockroach antigen have been known as a cause of allergic disease. German ockroach(Blattella germanica L.) was chosen because it has the highest distibution range and poulation density. To identify the common and specific antigens of adult and
larval
stage of german cockraoch, we made monoclonal antibodies which were confirmen by SDSPAGE and EITB. Anti-B. germanica antibody producing hydridomas were 24 among the total 960 wells. Only 4 hydridomas did not have cross reaction to other species
of
cockroach and house dust mites(Dermatophagoides farinae and D. Pteronyssius). SDS-PAGE revealed about 20 bands from 90Kd to 15Kd. EITB showed specific antigens at 60, 72 and 82Kd which were experimented by the culture supernatant of 4 selected
hydridomas. Especially 60Kd coincided with a band of immunized mouse sera.
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